Vyhodnocení přesnosti odhadu objemu Langerhansových ostrůvku z mikroskopických obrazů

Accuracy evaluation of Langerhans islet volume estimation from microscopic images

Typ dokumentu
diplomová práce
master thesis
Autor
Hlushak Hanna
Vedoucí práce
Kybic Jan
Oponent práce
Rožánek Martin
Studijní obor
Biomedicínské inženýrství
Studijní program
Biomedicínská a klinická technika (studium v angličtině)
Instituce přidělující hodnost
katedra biomedicínské techniky
Obhájeno
2016-06-22


Práva
A university thesis is a work protected by the Copyright Act. Extracts, copies and transcripts of the thesis are allowed for personal use only and at one?s own expense. The use of thesis should be in compliance with the Copyright Act http://www.mkcr.cz/assets/autorske-pravo/01-3982006.pdf and the citation ethics http://knihovny.cvut.cz/vychova/vskp.html
Vysokoškolská závěrečná práce je dílo chráněné autorským zákonem. Je možné pořizovat z něj na své náklady a pro svoji osobní potřebu výpisy, opisy a rozmnoženiny. Jeho využití musí být v souladu s autorským zákonem http://www.mkcr.cz/assets/autorske-pravo/01-3982006.pdf a citační etikou http://knihovny.cvut.cz/vychova/vskp.html
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For successful islet transplantation it is important to input sufficient quantity of islets, hence it is required to know the volume of the transplanted islets. Several methods for estimating the volume from 2D microscopy images are used (the Ricordi table, spherical and ellipsoid models). The aim of this work is to evaluate the accuracy of these methods. With the help of Optical Tomography microscope we made 401 projections of individual islets and created 3D models by image reconstruction. We calculated the individual islet volumes by the isotropic Fakir method and compared them with results from the evaluated 2D methods for 65 real islets and 35 synthetically generated islets. Ellipse fitting method for islet volume estimation performed by plugins of Fiji software was shown to be the most suitable among the evaluated 2D methods.
 
For successful islet transplantation it is important to input sufficient quantity of islets, hence it is required to know the volume of the transplanted islets. Several methods for estimating the volume from 2D microscopy images are used (the Ricordi table, spherical and ellipsoid models). The aim of this work is to evaluate the accuracy of these methods. With the help of Optical Tomography microscope we made 401 projections of individual islets and created 3D models by image reconstruction. We calculated the individual islet volumes by the isotropic Fakir method and compared them with results from the evaluated 2D methods for 65 real islets and 35 synthetically generated islets. Ellipse fitting method for islet volume estimation performed by plugins of Fiji software was shown to be the most suitable among the evaluated 2D methods.
 
URI
http://hdl.handle.net/10467/67590
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