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Analysis of Elemental Localization in Cells by Energy Dispersive X-Ray Spectroscopy

dc.contributor.advisorGiepmans Ben
dc.contributor.authorPirozzi Nicole
dc.date.accessioned2018-03-12T18:05:59Z
dc.date.available2018-03-12T18:05:59Z
dc.date.issued2016-05-20
dc.identifierKOS-695600290305
dc.identifier.urihttp://hdl.handle.net/10467/75426
dc.description.abstract1.Expose fixed rat pancreatic tissue and/or cultred cells to several elements that are absent or only in trace amounts present in mammals (may include As, Ba, Cd, Cr, Pd, Hg, Se, Ag, Co, Cu) at appropriate concentrations and time to reach inter/intracellular concentrations ?100 ppm 2.Further fixate the exposed tissue in preparation for scanning electron microscopy (SEM) 3. Perform energy dispersive x-ray spectroscopy (EDx) of tissue 4.Analyze spatial localization of exposed element and evaluation of success and potential 5. Study to be performed at Cell Biology Department, University Medical Center Groningen, The Netherlands, under supervision of Dr. Ben Giepmanscze
dc.description.abstractFeature identification of high-resolution electron microscopy images can be improved with the addition of elemental detection via energy dispersive x-ray spectroscopy (EDX). Fixed rat pancreas samples were incubated in eleven different metallic compounds then elemental composition was analyzed with Phenom-World Pro-X tabletop scanning electron microscope with elemental analysis. From analysis of elemental localization, it was determined that pre-embedding incubation of tissue with heavy metals is a viable method for staining. The most promising metals: hafnium, gadolinium, and samarium, should be further explored for elemental labeling by EDX.eng
dc.language.isoENG
dc.publisherČeské vysoké učení technické v Praze. Vypočetní a informační centrum.cze
dc.publisherCzech Technical University in Prague. Computing and Information Centre.eng
dc.rightsA university thesis is a work protected by the Copyright Act. Extracts, copies and transcripts of the thesis are allowed for personal use only and at one?s own expense. The use of thesis should be in compliance with the Copyright Act http://www.mkcr.cz/assets/autorske-pravo/01-3982006.pdf and the citation ethics http://knihovny.cvut.cz/vychova/vskp.html.eng
dc.rightsVysokoškolská závěrečná práce je dílo chráněné autorským zákonem. Je možné pořizovat z něj na své náklady a pro svoji osobní potřebu výpisy, opisy a rozmnoženiny. Jeho využití musí být v souladu s autorským zákonem http://www.mkcr.cz/assets/autorske-pravo/01-3982006.pdf a citační etikou http://knihovny.cvut.cz/vychova/vskp.html.cze
dc.subjectelectron microscopy,electron dispersive x-ray spectroscopy,biosciences,cell biology,pancreas,tissue,metal labeling,color electron microscopycze
dc.subjectelectron microscopy,electron dispersive x-ray spectroscopy,biosciences,cell biology,pancreas,tissue,metal labeling,color electron microscopyeng
dc.titleAnalýza lokalizace prvků v buňkách pomocí energiově disperzní rentgenové spektroskopiecze
dc.titleAnalysis of Elemental Localization in Cells by Energy Dispersive X-Ray Spectroscopyeng
dc.typeMAGISTERSKÁ PRÁCEcze
dc.typeMASTER'S THESISeng
dc.date.accepted2016-06-22
dc.contributor.refereePetrák Václav
theses.degree.disciplineBiomedicínské inženýrstvícze
theses.degree.grantorkatedra biomedicínské technikycze
theses.degree.programmeBiomedicínská a klinická technika (studium v angličtině)cze


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