QCM analýza nanočástic a molekul

Abstract

Sensors based on quartz crystal microbalances (QCM) have dominated research in recent years. They have produced excellent laboratory results in analyzing the mass as well as the conformation of nanoparticles and molecules. The technology is constantly developed and widely used in various fields of chemistry, physics and biology. Here, we investigated a novel concept based on the use of nanodiamonds on QCM and explored the performance of nanodiamonds on QCM sensors, which helps in expanding the application of nanodiamonds in biosensing and exploring their effectiveness in QCM setups. We established a procedure and suitable parameters for the QCM sensor measurement. 5 MHz opensource QCM sensor system was used for building up the sensor assay for detection of cortisol via antigen (Ag)-antibody (Ab) reactions. Optical pictures after each functionalization step as well as scanning electron microscopy (SEM) pictures before and after the experiment were observed and compared. The values of amplitude, phase, resonance frequency, and dissipation were acquired in dry and water environment. The main evaluated QCM parameters were changes in resonance frequency (Δf) and simultaneous changes in QCM energy dissipation (ΔD), corresponding to changes in mass and morphology of the investigated sensor structure. Thereby we identified sensor assay build up, its functioning in dry and water conditions as well as potential issues such as partial release of nanodiamonds during the chemical treatments.

Sensors based on quartz crystal microbalances (QCM) have dominated research in recent years. They have produced excellent laboratory results in analyzing the mass as well as the conformation of nanoparticles and molecules. The technology is constantly developed and widely used in various fields of chemistry, physics and biology. Here, we investigated a novel concept based on the use of nanodiamonds on QCM and explored the performance of nanodiamonds on QCM sensors, which helps in expanding the application of nanodiamonds in biosensing and exploring their effectiveness in QCM setups. We established a procedure and suitable parameters for the QCM sensor measurement. 5 MHz opensource QCM sensor system was used for building up the sensor assay for detection of cortisol via antigen (Ag)-antibody (Ab) reactions. Optical pictures after each functionalization step as well as scanning electron microscopy (SEM) pictures before and after the experiment were observed and compared. The values of amplitude, phase, resonance frequency, and dissipation were acquired in dry and water environment. The main evaluated QCM parameters were changes in resonance frequency (Δf) and simultaneous changes in QCM energy dissipation (ΔD), corresponding to changes in mass and morphology of the investigated sensor structure. Thereby we identified sensor assay build up, its functioning in dry and water conditions as well as potential issues such as partial release of nanodiamonds during the chemical treatments.