Kultivované neurony jako platforma pro hodnocení psychoaktivních látek
Cultured neurons as a platform for assessing psychoactive substances
Typ dokumentu
diplomová prácemaster thesis
Autor
Kateřina Nevšímalová
Vedoucí práce
Petrák Václav
Oponent práce
Kliment Radim
Studijní obor
NanotechnologieStudijní program
Biomedicínská a klinická informatikaInstituce přidělující hodnost
katedra biomedicínské informatikyObhájeno
2023-06-21Práva
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The aim of this study was to assess the effects of the neurostimulation substances MDMA and 25CN-NBOMe on the nervous system by stimulating a cultivated hippocampal neural line. We investigated whether the effects of these substances are dose-dependent, hence several different concentrations were tested. In vitro microelectrode arrays were used to measure electrical neural signals. Changes in neuronal activity were assessed based on seven electrophysiological parameters such as Mean Firing Rate (MFR), Inter Spike Interval (ISI), and others. After stimulating neurons with 1µM MDMA, we observed a significant change in MBR, which dropped almost to zero, while MFR and network synchronization did not change significantly. We observed a significant inhibitory effect when stimulating with 25CN-NBOMe at concentrations of 3 mg/L and 30 mg/L, at which both MFR and MBR dropped almost to zero. However, it is strange that this significant effect of reducing nerve activity was not caused by the 25CN-NBOMe stimulation at a concentration of 10 mg/L. To confirm these results, it would be necessary to repeat these experiments. The aim of this study was to assess the effects of the neurostimulation substances MDMA and 25CN-NBOMe on the nervous system by stimulating a cultivated hippocampal neural line. We investigated whether the effects of these substances are dose-dependent, hence several different concentrations were tested. In vitro microelectrode arrays were used to measure electrical neural signals. Changes in neuronal activity were assessed based on seven electrophysiological parameters such as Mean Firing Rate (MFR), Inter Spike Interval (ISI), and others. After stimulating neurons with 1µM MDMA, we observed a significant change in MBR, which dropped almost to zero, while MFR and network synchronization did not change significantly. We observed a significant inhibitory effect when stimulating with 25CN-NBOMe at concentrations of 3 mg/L and 30 mg/L, at which both MFR and MBR dropped almost to zero. However, it is strange that this significant effect of reducing nerve activity was not caused by the 25CN-NBOMe stimulation at a concentration of 10 mg/L. To confirm these results, it would be necessary to repeat these experiments.