Experimentální studie subletální expozice baktérií nanočásticemi a možný vývoj jejich rezistence.
Experimental study into sub-lethal exposure of bacteria to nanoparticles and the possible development of resistance
Typ dokumentu
bakalářská prácebachelor thesis
Autor
Mohammed Fadel
Vedoucí práce
Rutherford David
Oponent práce
Galář Pavel
Studijní program
Electrical Engineering and Computer ScienceInstituce přidělující hodnost
katedra fyzikyPráva
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Zinc nanoparticles are the most common nanotechnology NPs that provides a major contribution to the production of large-scale consumer goods. In developed nations, hazardous microbe infections pose a major public health issue, largely due to less awareness of antibiotic usage and vaccination. The creation of compounds to treat infection is essential to handle certain pathogenic bacteria, such as multiple drug resistance lines. To research the resistance to hazardous microbial strains, scientists use nanoparticles (NPs). These NPs are also used to treat diseases like cancer that kill cells with DNA. The thesis aimed to study the effects of nano ZnO and micro ZnO against E. coli, by analyzing cell viability. Zinc oxide nanoparticle was characterized and was exposed to the E. coli. The cell viability of E. coli concentration between 100 to 1000 μg/mL was observed. The growth concentration nano and micro exposure was observed in T0 and T24. The exposure effects indicate that stock solution exhibited a higher impact on its cell membrane viability as the concentration tends towards 0 μg/mL. Viable concentrations are expressed in terms of ZnO concentration and the length of unit time in stock solution. In conclusion, the research findings show the importance of incorporating a wide range of possible endpoints to determine NPs toxicity as it provides an in-depth assessment of discrete impacts, otherwise not revealed by a narrow choice of endpoints. Zinc nanoparticles are the most common nanotechnology NPs that provides a major contribution to the production of large-scale consumer goods. In developed nations, hazardous microbe infections pose a major public health issue, largely due to less awareness of antibiotic usage and vaccination. The creation of compounds to treat infection is essential to handle certain pathogenic bacteria, such as multiple drug resistance lines. To research the resistance to hazardous microbial strains, scientists use nanoparticles (NPs). These NPs are also used to treat diseases like cancer that kill cells with DNA. The thesis aimed to study the effects of nano ZnO and micro ZnO against E. coli, by analyzing cell viability. Zinc oxide nanoparticle was characterized and was exposed to the E. coli. The cell viability of E. coli concentration between 100 to 1000 μg/mL was observed. The growth concentration nano and micro exposure was observed in T0 and T24. The exposure effects indicate that stock solution exhibited a higher impact on its cell membrane viability as the concentration tends towards 0 μg/mL. Viable concentrations are expressed in terms of ZnO concentration and the length of unit time in stock solution. In conclusion, the research findings show the importance of incorporating a wide range of possible endpoints to determine NPs toxicity as it provides an in-depth assessment of discrete impacts, otherwise not revealed by a narrow choice of endpoints.