Návrh a realizace principu bez značkové detekce DNA molekul pomocí kvantových elektronových přechodů NV center, vytvořených v diamantovém nanosensoru

Abstract

DNA diagnostics became indispensable part of biochemical analytic tools today. Further challenges lay in development of sensors which can detect significantly reduced volume of analyte, operate with enhanced sensitivity and analyse larger DNA arrays. In the heart of development of such sensor sis search for novel sensing principles that would enable a significant improvement in precision, speed and resolution. I tis anticipated that such principles might lead to novel medical diagnosis and genetics with a possibility to detect variety of diseases or gene mutations. Diamond, as wide bandgap semiconductor is an attractive materiál for developing novel devices for biosensing applications due to its excellent physical and chemáical properties. In this doctoral work was devoted to development of novel label free DNA microfluidic biosensor based on quantum sensing principles. We propose a scheme in which so called colour centres in diamond, e.g. a lattice point defects, work as molecular sensors. Our design employs specifically nitrogen vacancy NV centre chargé state detection in diamond combining electrochemical detection with NV centre chargé state optical readout.DNA diagnostics became indispensable part of biochemical analytic tools today. Further challenges lay in development of sensors which can detect significantly reduced volume of analyte, operate with enhanced sensitivity and analyse larger DNA arrays. In the heart of development of such sensor sis search for novel sensing principles that would enable a significant improvement in precision, speed and resolution. I tis anticipated that such principles might lead to novel medical diagnosis and genetics with a possibility to detect variety of diseases or gene mutations. Diamond, as wide bandgap semiconductor is an attractive materiál for developing novel devices for biosensing applications due to its excellent physical and chemáical properties. In this doctoral work was devoted to development of novel label free DNA microfluidic biosensor based on quantum sensing principles. We propose a scheme in which so called colour centres in diamond, e.g. a lattice point defects, work as molecular sensors. Our design employs specifically nitrogen vacancy NV centre chargé state detection in diamond combining electrochemical detection with NV centre chargé state optical readout.