KOZAK, H., et al. Infrared Absorption Spectroscopy of Albumin Binding with Amine-Containing Plasma Polymer Coatings on Nanoporous Diamond Surfaces. Langmuir. 2019, 35(43), 13844-13852. ISSN 0743-7463. DOI 10.1021/acs.langmuir.9b02327.
Nanocrystalline diamond (NCD) layers functionalized with amine-containing functional groups have generated considerable interest as biocompatible substrates for attachment of biomolecules and cells with a view to biosensor and tissue engineering applications. Here we prepare nanoporous diamond layers with the surfaces modified by hydrogen plasma, oxygen plasma, and conformal 7 nm amine-containing plasma polymer (PP). Immobilization of bovine serum albumin (BSA) molecules is characterized on such surfaces. Grazing angle reflectance infrared spectroscopy as well as X-ray photoelectron spectroscopy show that concentration of amine-containing bonds after BSA exposure depends on the type of NCD surface modification. AFM measurements reveal that BSA proteins are physisorbed on H- and O-terminated diamond surfaces in different thicknesses and morphology. When the diamond layers are coated with the amine-containing PP, BSA molecules assume similar thickness and morphology, and their adhesion is significantly increased on both types of the diamond surfaces.
eng
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application/pdf
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eng
dc.publisher
American Chemical Society
dc.relation.ispartof
Langmuir
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CONDUCTIVITY
eng
dc.subject
TEMPERATURE
eng
dc.subject
infrared spectroscopy
eng
dc.subject
diamond
eng
dc.subject
proteins
eng
dc.subject
plasma polymer
eng
dc.title
Infrared Absorption Spectroscopy of Albumin Binding with Amine-Containing Plasma Polymer Coatings on Nanoporous Diamond Surfaces