Nanogelová platforma pro podávání hydrofobních léčiv na bázi cyklodextrinů

Abstract

A key problem in healthcare is the predominance of bacterial infections, resistance development through environmental build-up. While novel antimicrobials have been developed, there is a need for nanoscale drug vectors for these agents. The investigation aimed to create a novel cyclodextrin based nanogel for the primary purpose of encapsulating a photosensitive azobenzene-based antimicrobial drug. This drug has ability to self-regulate deactivation within the body, mitigating concerns of antimicrobial build-up and associated resistance. A coprecipitation polymerisation method was to combine N-isopropylmethacrylamide and a functionalized derivative of a β-CD. Issues were encountered with characterisation techniques and loading of azobenzene. The loading capacity was found to be equivalent to the atypical binding capacity through UV/VIS spectroscopy. However, it was inconclusive as to whether cyclodextrin had been successfully incorporated and a novel nanogel had been successfully synthesised. An alternative synthesis method was employed using NIPAM which showed successful inclusion. This nanogel complex was synthesised using an EDC/NHS crosslinking based approach. Additionally, further investigation of cyclodextrin nanogels of varied molar equivalence would be needed to verify the success. Along with Nile Red, repeated FTIR and additional loading experiments could evaluate the potential success of co- polymerisation. Both techniques could then be further tested for loading capacity and triggered release and their characteristic capacity and behaviour compared.A key problem in healthcare is the predominance of bacterial infections, resistance development through environmental build-up. While novel antimicrobials have been developed, there is a need for nanoscale drug vectors for these agents. The investigation aimed to create a novel cyclodextrin based nanogel for the primary purpose of encapsulating a photosensitive azobenzene-based antimicrobial drug. This drug has ability to self-regulate deactivation within the body, mitigating concerns of antimicrobial build-up and associated resistance. A coprecipitation polymerisation method was to combine N-isopropylmethacrylamide and a functionalized derivative of a β-CD. Issues were encountered with characterisation techniques and loading of azobenzene. The loading capacity was found to be equivalent to the atypical binding capacity through UV/VIS spectroscopy. However, it was inconclusive as to whether cyclodextrin had been successfully incorporated and a novel nanogel had been successfully synthesised. An alternative synthesis method was employed using NIPAM which showed successful inclusion. This nanogel complex was synthesised using an EDC/NHS crosslinking based approach. Additionally, further investigation of cyclodextrin nanogels of varied molar equivalence would be needed to verify the success. Along with Nile Red, repeated FTIR and additional loading experiments could evaluate the potential success of co- polymerisation. Both techniques could then be further tested for loading capacity and triggered release and their characteristic capacity and behaviour compared.